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PNF Preventive Nutrition and Food Science

Open Access


ISSN 2287-8602
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Original

Article

Original

Prev Nutr Food Sci 2021; 26(3): 330-337

Published online September 30, 2021 https://doi.org/10.3746/pnf.2021.26.3.330

Copyright © The Korean Society of Food Science and Nutrition.

Onion Peel Extract Inhibits Cancer Cell Growth and Progression through the Roles of L1CAM, NF-κB, and Angiogenesis in HT-29 Colorectal Cancer Cells

Tamonwan Uttarawichien1* , Wilunplus Khumsri1* , Prasit Suwannalert1 , Nathawut Sibmooh2 , and Witchuda Payuhakrit1

1Department of Pathobiology, Faculty of Science, Mahidol University, Bangkok 10400, Thailand
2Chakri Naruebodindra Medical Institute, Faculty of Medicine Ramathibodi Hospital, Mahidol University, Samut Prakan 10540, Thailand

Correspondence to:Witchuda Payuhakrit, Tel: +66-2201-5550, E-mail: witchuda.pay@mahidol.ac.th
*These authors contributed equally to this work.

Received: March 24, 2021; Revised: June 22, 2021; Accepted: July 8, 2021

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Colorectal cancer (CRC) is an aggressive malignancy. Critical mechanisms that support CRC progression include cell migration, invasion, metastasis, and angiogenesis, which is associated with L1 cell adhesion molecule (L1CAM) and nuclear factor-kappa B (NF-κB) signaling pathways. In this study, viability of HT-29 cells and human umbilical vein endothelial cells (HUVECs) was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assays, and cell apoptosis was investigated by flow cytometry assays. HT-29 cell migration and invasion were observed by wound healing and Transwell invasion assays, respectively, and tube formation of HUVECs was observed by tubulogenesis assays. L1CAM and NF-κB protein expressions in HT-29 cells treated with onion peel extract were determined by indirect immunofluorescence. Results showed that high dose treatments of onion peel extract inhibited cell viability of both HT-29 cells and HUVECs, induced HT-29 cell apoptosis, and inhibited HT-29 cell migration and invasion. Moreover, onion peel extract decreased total HUVEC tube length and, at a concentration of 10 μg/mL, showed potential to downregulate L1CAM and NF-κB. In conclusion, onion peel extract inhibits HT-29 cell growth, migration, and invasion through suppressing pathways related to angiogenesis downstream of L1CAM-activated NF-κB.

Keywords: angiogenesis, colorectal cancer, L1CAM, NF-κB, onion peel extract