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PNF Preventive Nutrition and Food Science

Open Access


ISSN 2287-8602
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Original

Article

Original

Prev Nutr Food Sci 2021; 26(3): 315-320

Published online September 30, 2021 https://doi.org/10.3746/pnf.2021.26.3.315

Copyright © The Korean Society of Food Science and Nutrition.

Evaluation of Safety through Acute and Subacute Tests of Galacto-Oligosaccharide (GOS)

Youngjin Baek1,2 , Yejin Ahn1 , Jungcheul Shin2 , Hyung Joo Suh1 , and Kyungae Jo1

1Department of Integrated Biomedical and Life Science, Graduate School, Korea University, Seoul 02841, Korea
2Department of R&D, Neo Cremar Co., Ltd., Seoul 05702, Korea

Correspondence to:Kyungae Jo, Tel: +82-2-940-2764, E-mail: kyungae11@korea.ac.kr

Received: March 9, 2021; Revised: April 23, 2021; Accepted: June 4, 2021

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Acute and subacute toxicity tests were undertaken on a novel galacto-oligosaccharide (GOS) produced from lactose by β-galactosidase derived from Bacillus circulans. Toxicity was evaluated by single dose oral administration (5,000 mg/kg) and was repeated at day 28 (1,000 mg/kg) in male and female Sprague-Dawley rats. In acute toxicity tests, the protein levels of male rats administered GOS showed a significant difference from controls, but remained within the normal range. There were no GOS-related changes in clinical symptoms, weight, food intake, hematology, blood chemistry, relative organ weight, or severe pathology in rats treated with GOS compared with controls. The no observed adverse effect level of GOS was at least 1,000 mg/kg/d in both male and female rats. Bovine-specific genes were not detected in GOS 70%-based products (NeoGOS-P70, NeoGOS-L70, and organic GOS), indirectly showing the absence of an allergen and that products containing GOS 70% are non-toxic and allergen-free.

Keywords: acute toxicity, galacto-oligosaccharide, subacute toxicity