HPLC-UV conditions of sesamol, sesamin, and sesamolin analysis
Instrument (HPLC-UV) | Hitachi 5000 chromaster (Hitachi LTD) |
---|---|
Column | Agilent Zorbax Eclipse XDB-C18 column (5 μm, 150 mm×4.6 mm) |
Column oven temperature | 30°C |
Injection volume | 20 μL |
Flow rate | 0.7 mL/min |
Mobile phase | Distilled water:MeOH (3:7, v/v) |
HPLC-UV, high-performance liquid chromatography-ultraviolet.