Validation methods
Parameter | Evaluation method | Result |
---|---|---|
Specificity | Obtained the HPLC analysis retention time | ∙Retention time for both: approximately 21 min ∙No peak interference phenomena were observed ∙Quercitrin peak spectrum in the standard solution and sample (DJE) matches |
Linearity | Confirmed with six concentrations of the standard substance | ∙A range of 2.5-15.0 μg/mL was established to assess the accuracy and precision ∙Average coefficient of determination (R2): 0.99975 |
Accuracy | Evaluated the recovery rate by spiking the samples with the standard substance at three different concentrations | Recovery rate: 89.02%-99.30% %RSD range: 0.50%-5.95% |
Precision | Evaluated reproducibility by having multiple analysts in each laboratory analyze the same sample using the same instrumentation. Confirmed repeatability by having an analyst assess the content of three or more samples |
∙ReproducibilityQuercitrin content: 0.316-0.344 mg/g% RSD range: 0.835%-1.379% ∙RepeatabilityQuercitrin content: 0.290-0.326 mg/g%RSD range: 0.94%-3.21% |
HPLC, high-performance liquid chromatography; DJE, Dangjo extract; RSD, relative standard deviation.