Fig. 2. OGT and MOF regulate the proliferation of MOVAS and fibroblast cell lines. (A) Effects of OGT siRNA transfection and (B) MOF siRNA transfection. (C) Quantification of cell viability in siOGT-transfected MOVAS cells. (D) Quantification of cell viability in siMOF-transfected MOVAS cells. (E) Quantification of the cell number in siOGT- or siMOF-transfected NHDF cell line. (F) Immunofluorescence staining of OGT (red) in the NHDF cell line or siOGT-transfected NHDF cells with/without Thiamet G treatment. (G) Quantification of OGT (red) in the NHDF cell line or siOGT-transfected NHDF cells with/without Thiamet G (Thia) treatment. CON, n=32; CON+Thia, n=31; siOGT, n=62; siOGT+Thia, n=70. Data are presented as the meanĀ±SD. **P<0.01, ***P<0.001, ****P<0.0001. ns, non-significant. n indicates the cell numbers. OGT, O-GlcNAc transferase; TF, transfection; MOF, males absent on the first; MOVAS, mouse vascular smooth muscle cells; N.C., negative control; CON, control; NHDF, normal human dermal fibroblast.
© Prev. Nutr. Food Sci.