Quantitative real-time polymerase chain reaction (qRT-PCR) information for viral gene expression analysis
Virus | Primer sequences (5’→3’) | Target gene | qRT-PCR condition | |
---|---|---|---|---|
H1N1 | F: | TCGCCGACTATGAGGAACTGAGGGA | Hemagglutinin | (95°C-15 s/61°C-20 s/72°C-60 s); 50 cycles |
R: | TGGTATCCCCGGGTTCCAGCAGAGT | |||
IVB | F: | AAAACAAATGCTCTGCGCCCCAAC | Nucleoprotein | (95°C-15 s/63°C-20 s/72°C-45 s); 50 cycles |
R: | CGTCTCCACCTACTTCATTCCCCCC | |||
RSV-A | F: | TGACCCATTAGTGTTCCCCTCTGATGAAT | Fusion protein | (95°C-15 s/64°C-20 s/72°C-30 s); 45 cycles |
R: | CTTCTGGCCTTACAGTATAGGAGCAGT |
IVB, influenza B virus; RSV-A, respiratory syncytial virus subtype A.