Table. 1. Table 1

Primer sequences for RT-qPCR

Gene name	Sequences of forward and reverse primer (5’-3’)		Tm (°C)
COX-2 (cyclooxygenase-2)	Forward	AGCCCATTGAACCTGGACTG	59.0
COX-2 (cyclooxygenase-2)	Reverse	ACCCAATCAGCGTTTCTCGT	59.0
GAPDH (glyceraldehyde 3-phosphate dehydrogenase)	Forward	AAGGTCATCCCAGAGCTGAA	59.5
GAPDH (glyceraldehyde 3-phosphate dehydrogenase)	Reverse	CTGCTTCACCACCTTCTTGA	59.5
IL-6 (interleukin-6)	Forward	AGTCCTTCCTACCCCAATTTCC	59.5
IL-6 (interleukin-6)	Reverse	TGGTCTTGGTCCTTAGCCAC	59.5
IL-10 (interleukin-10)	Forward	TGCCTGCTCTTACTAACTGG	59.0
IL-10 (interleukin-10)	Reverse	CTCTAGGAGCATGTGGCTCTG	59.0
iNOS (inducible nitric oxide synthase)	Forward	AGAACGGAGAACGGAGAACG	58.9
iNOS (inducible nitric oxide synthase)	Reverse	GAAGAGAAACTTCCAGGGGCA	58.9
TNF-α (tumor necrosis factor-α)	Forward	AAAGACACCATGAGCACAGAAAGC	62.0
TNF-α (tumor necrosis factor-α)	Reverse	GCCACAAGCAGGAATGAGAAGAG	62.0

RT-qPCR, real-time quantitative polymerase chain reaction; Tm, temperature.

Prev Nutr Food Sci 2024;29:301~310 https://doi.org/10.3746/pnf.2024.29.3.301