Fig. 4. Inhibition of inflammatory cytokine secretion by SGWE or SGEE treatment in ox-LDL- and LPS-induced foam cells. THP-1 foam cells were pretreated with different concentrations of SGWE (0－250 μM) for 48 h. (A－D) The secretion of IL-6 and TNF-α was measured using an ELISA kit. The expression levels of TNF-α and COX-2 were measured using (E and F) immunoblotting. Cells were harvested, and the expression of COX-2 (G and H) and TNF-α (I and J) mRNA in ox-LDL- and LPS-induced foam cells was evaluated. Data are presented as the mean±standard deviation. Different letters indicate significant differences (P<0.05), as determined by Duncan’s multiple range test. SGWE, Siegesbeckia glabrescens water extract; SGEE, S. glabrescens ethanol extract; ox-LDL, oxidized low density lipoprotein; LPS, lipopolysaccharides; IL-6, interleukin 6; TNF-α, tumor necrosis factor-alpha; ELISA, enzyme-linked immunosorbent assay; COX-2, cyclooxygenase-2.

Prev Nutr Food Sci 2024;29:288~300 https://doi.org/10.3746/pnf.2024.29.3.288