Fig. 3. Upregulation of ABCA1, LXRα, and PPARγ expression by SGWE or SGEE treatment in THP-1 foam cells. (A-D) The protein expression levels of ABCA1, LXRα, and PPARγ were determined using immunoblotting. Cells were harvested, and the expression of the mRNA levels of ABCA1 (E and F), LXRα (G and H), and PPARγ (I and J) in ox-LDL- and LPS-induced foam cells were evaluated. Data are presented as the mean±standard deviation. Data were analyzed by applying the 2—ΔΔCT method. Different letters indicate significant differences (P<0.05), as determined by Duncan’s multiple range test. ABCA1, ATP binding cassette transporter A1; LXRα, liver-X-receptor αlpha; PPARγ, peroxisome proliferator-activated receptor gamma; SGWE, Siegesbeckia glabrescens water extract; SGEE, S. glabrescens ethanol extract; ox-LDL, oxidized low density lipoprotein; LPS, lipopolysaccharides.
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