Fig. 2. Inhibition of SR-A1, CD36, and LOX-1 expression by SGWE and SGEE treatment in THP-1 foam cells. (A and B) The protein expression levels of SR-A1, CD36, and LOX-1 were determined using immunoblotting. (C-H) The relative mRNA expression levels of CD36 (C and D), LOX-1 (E and F), and SR-A1 (G and H) were measured after normalization against β-actin mRNA expression. The data are expressed relative to the mRNA levels found in untreated cells, which were arbitrarily defined as one. Experiments were performed at least in triplicate, and the results are presented as the mean±standard deviation. Data were analyzed by applying the 2—ΔΔCT method. Different letters indicate significant differences (P<0.05), as determined by Duncan’s multiple range test. SR-A1, scavenger receptor class A1; CD36, cluster of differentiation 36; LOX-1, lectin-like oxidized low-density lipoprotein receptor-1; SGWE, Siegesbeckia glabrescens water extract; SGEE, S. glabrescens ethanol extract; LPS, lipopolysaccharides; ox-LDL, oxidized low density lipoprotein.
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