Fig. 5. Effects of Agastache rugosa extract (ARE) and tilianin on oxidative stress in C2C12 myotubes. C2C12 myotubes were simultaneously exposed to tumor necrosis factor-α (TNF-α, 50 ng/mL) along with ARE (40 and 80 μg/mL) or tilianin (20 and 40 μM). Reverse transcription polymerase chain reaction was used to determine the mRNA expression levels of catalase, SOD, and GPx in response to (A) ARE and (B) tilianin. β-Actin served as the internal control. Experimental results were presented as the mean±SD. Significant differences were assessed using Duncan’s multiple range test. ##P<0.01 (control group vs. TNF-α group); *P<0.05, **P<0.01 (TNF-α group vs. ARE or tilianin group).
© Prev. Nutr. Food Sci.