Table. 1. Table 1

Box-Behnken design parameters for ultrasound-assisted extraction of green leafy vegetable using response surface methodology

Run	Standard	Independent variable			Dependent variable
Run	Standard	Factor X₁ Ethanol concentration (%)	Factor X₂ Amplitude (%)	Factor X₃ Sonication time (min)	Yield (%)	α-Glucosidase inhibitory activity (mmol ACE/g extract)
1	2	80	40	20	20.01±0.18^d	1,161.74±27.70^b
2	1	0	40	20	25.20±2.44^abc	1,070.39±60.08^bcd
3	10	40	80	10	27.99±2.42^a	1,045.24±39.03^d
4	12	40	80	30	25.70±2.15^abc	1,460.96±16.17^a
5	8	80	60	30	17.45±1.28^d	1,432.46±76.72^a
6	14	40	60	20	25.50±1.70^abc	964.46±59.73^de
7	15	40	60	20	26.49±1.34^abc	919.42±63.20^e
8	4	80	80	20	19.07±2.43^d	1,455.93±80.55^a
9	6	80	60	10	18.03±0.72^d	1,053.10±53.71^cd
10	5	0	60	10	27.90±2.20^ab	974.00±26.41^de
11	3	0	80	20	24.46±2.40^abc	1,059.49±53.77^cd
12	11	40	40	30	26.37±1.41^abc	1,150.01±66.64^bc
13	9	40	40	10	23.89±1.16^c	998.31±57.90^de
14	7	0	60	30	24.36±2.35^bc	989.66±49.17^de
15	13	40	60	20	25.22±1.38^abc	969.72±56.06^de

Values are presented as mean±SD of three technical replicates (i.e., n=3).

Different superscripts in the same column (a-e) indicate statistically significant differences (P<0.05).

ACE, acarbose equivalent.

Prev Nutr Food Sci 2024;29:47~62 https://doi.org/10.3746/pnf.2024.29.1.47