Fig. 3. Effect of harmine hydrochloride (HMH) on cell cycle progression. (A) After treating cells with 0, 12.5, 25, and 50 μM of HMH for 24 h, flow cytometric analyses were performed on cells stained with propidium iodide to determine cell cycle progression. (B) Cumulative cell distribution in the Sub-G1, G0/G1, S, and G2/M phases of the cell cycle after HMH treatment. (C) The expression levels of G2/M-related proteins such as p53, p21, p-cdc2, cdc2, cyclin B1, and Rb were determined using Western blot analysis. β-Actin was used as an internal control.

Prev Nutr Food Sci 2023;28:436~443 https://doi.org/10.3746/pnf.2023.28.4.436