Fig. 7. Effect of ursolic acid (UA) on ERK and PI3K/AKT signaling pathways in MCF-7 cell-derived breast cancer stem (BCS)-like cells. (A) Representative blots showing protein expression of ERK and PI3K/AKT in MCF-7 cell-derived BCS-like cells treated with UA at 5, 10, and 20 μM for 24 h. (B and C) Cells were treated with UA in combination with the ERK inhibitor PD98059 or the AKT inhibitor LY294002, and levels of p-ERK or p-AKT were detected by Western blotting. (D and E) Band intensities were normalized to total ERK or AKT. Results are presented as mean±SD. **P<0.01 and ***P<0.001 compared to total ERK or AKT.