Fig. 3. Effect of ursolic acid (UA) on migration, invasion, and colony formation of MCF-7 cells. (A) Cells were grown to confluence in 6-well plates, scratch-wounded, and treated with the various concentrations of UA. (C) The filter membranes were coated with Matrigel and 0.02% gelatin. MCF-7 cells were treated UA for 48 h. (E) Cells were seeded onto 6-well cell culture plates and stained with 0.5% crystal violet. (B, D, and F) The wound area, invading cell number, and colony number were measured using ImageJ. Values are presented as mean±SD. *P<0.05 and ***P<0.001 vs. control.