Fig. 3. Effect of test substance on mRNA expression of collagen type I in mice skin. Total RNA isolated from mice skin was used to amplify the genes. Equal quantities of cDNA was used for the reverse transcriptase-PCR reaction and equal quantities of the amplified product was used to load the wells. For normalization, glyceraldehyde 3-phosphate dehydrogenase (GAPDH) expression separately determined of the same cDNA. L (protein ladder), 1 (cell control; G1), 2 (path control; G3), 3 (standard control; G4), 4 (sham control; G2) 5 (test A high dose; G6), 6 (test A low dose; G5), 7 (test B high dose; G8), and 8 (test B low dose; G7).

Prev Nutr Food Sci 2021;26:425~433 https://doi.org/10.3746/pnf.2021.26.4.425