Primer sequences used in reverse transcription-polymerase chain reaction analysis
Origin | Gene | Direction | Sequence (5’→3’) |
---|---|---|---|
Rat | Atrogin-1 | Forward | CCCTGAGTGGCATCGCCCAA |
Reverse | AGGTCCCGCCCATCGCTCA | ||
MuRF1 | Forward | CCAACGACATCTTCCAGGCT | |
Reverse | TGGTGGCTGTTTTCCTTGGT | ||
β-Actin | Forward | CTGTGTGGATTGGTGGCTCTAT | |
Reverse | GTGTAAAACGCAGCTCAGTAACA | ||
Mouse | Atrogin-1 | Forward | CAGTGAGCCCTGCCATAACA |
Reverse | TCCCAAGTATGGAGCAGGGA | ||
MuRF1 | Forward | GTCTGGAGGTCGTTTCCGTT | |
Reverse | AGCAAGTAGGCACCTCACAC | ||
β-Actin | Forward | TTACCAACTGGGACGACATG | |
Reverse | ATACAGGGACAGCACAGCCT |